Introduction
Objective
Report
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Introduction
A large-scale EST sequencing project has been undertaken for the purpose of gene discovery in the black tiger shrimp Penaeus monodon. It is a collaborative project involves Thai researchers from three universities and a research institute supported by Thailand National Center for Genetic Engineering and Biotechnology (BIOTEC). The project aims to generate approximately 30,000 EST clones from different tissues of shrimp under normal and infection or stress conditions to identify tissue-specific genes and genes responded to infection and stress. The collection of unique ESTs will be used for study of gene expression profiles of various phenotypes of interest. In addition, sequence information stored in the shrimp EST database will provide a useful genetic resource for identification of genetic markers i.e. microsatellite and single nucleotide polymorphism (SNP) markers for use in marker-assisted selection and the genetic linkage map. Knowledge from these genomic researches can be applied to the shrimp industry for increasing culture efficiency and sustainability.
Objective
Objectives of the project
To establish and provide a well-characterize shrimp EST resource for genomic studies of Penaeus monodon.
To develop the database of shrimp EST sequences and Gene Index for gene finding, gene characterization and EST data mining.
To develop EST markers as well as microsatellite and SNP markers for use in marker assisted selection of Penaeus monodon stocks/lines.
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